- Cell Line: NCI-H211
- Synonyms:
- NCI-H211
- NCIH211
- H211
- H-211
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell Line
- Description: Established from the bone marrow (metastatic site) of a 50-year-old, Caucasian female patient with Small Cell Lung Carcinoma, Stage E (Neuroendocrine Tumor)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 50 years
- Ethnicity: Caucasian
- Gender: Female
- Tissue (origin): Lung
- Tissue (isolation): Bone marrow (metastatic site)
- Disease:
- Small Cell Lung Carcinoma/Lung Neuroendocrine Tumor
- Primary/Metastasis: Metastatic
- Small Cell Lung Carcinoma/Lung Neuroendocrine Tumor
- Year of origin: 1980
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Aggregates in suspension
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- TP53 (R248Q mutation, Likely Oncogenic, Likely Loss-of-function)
- For information, please visit
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Harvesting time:-
- Split ratio: 1:2 to 1:5
- Medium Renewal: 2 to 3 times per week
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- AcceGen: Product link
- Cytion: Product link
- References
- Images:
- NCI-H82 cell image link (Source: DSMZ)
- NCI-H82 cell image link (Source: DSMZ)
- NCI-H82 cell image link (Source: DSMZ)
- NCI-H82 cell image link (Source: DSMZ)
- NCI-H82 cell image link (Source: Cytion)
- NCI-H82 cell image link (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
