- Cell Line: NCI-H446
- Synonyms:
- NCI-H446
- NCIH446
- H446
- H-446
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Epithelial Cell Line
- Description: Established from the Pleural effusion (metastatic site) of a male 61-year-old Caucasian patient with Small Cell Lung Carcinoma (Neuroendocrine Tumor)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 61 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion (metastatic site)
- Disease:
- Small Cell Lung Carcinoma/Lung Neuroendocrine Tumor
- Primary/Metastasis: Metastatic
- Small Cell Lung Carcinoma/Lung Neuroendocrine Tumor
- Year of origin: 1982
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Mixed: Adherent and suspension
- Life span: Infinite (continuous culture)
- Karyotype: Hypertriploid, modal chromosome number: 74
- Doubling time:-
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity: Yes, in nude mice
- Gene expression:
- Cells express neuron-specific enolase and the brain isoenzyme of creatine kinase
- Sequence variations:
- 20-fold amplification of C-myc DNA sequences and 15-fold increase in c-myc RNA relative to normal cells
- TP53 (G154V mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- FBXW7 (K11Q mutation, Likely Oncogenic, Likely Loss-of-function)
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- 20-fold amplification of C-myc DNA sequences and 15-fold increase in c-myc RNA relative to normal cells
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:9
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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