Cell Line: SW-13

  • Cell Line: SW-13
  • Synonyms:
    • SW-13
    • SW 13
  • Culture Type: Cell line
  • Keywords:  Human Adrenal Cortex Carcinoma Cell Line
  • Description: Established from the adrenal gland and cortex of a Caucasian, 55-year-old, female patient with Adrenal Cortex Carcinoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 55 years
      • Ethnicity: Caucasian
      • Gender: Female
    • Tissue (origin): Adrenal cortex
    • Disease: Adrenal Cortex Carcinoma
    • Primary/Metastasis: Primary
    • Collection site:-
    • Year of origin: 1971
  • Cell line features:
    • Cell Type: Epithelial
    • Morphology: Epithelial-like
    • Growth mode: Adherent
    • Life span: Infinite (continuous culture)
    • Karyotype: Modal number = 63, Range = 45 to 65
    • Doubling time:-
    • Microsatellite instability: stable
    • Special features: Electron microscopic studies show many bulb gap junctions (BGJ)
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:
    • Sequence variations:
      • TP53 (H193Y mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
      • For information, please visit
        • https://depmap.org/portal/cell_line/ACH-001401?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-001401?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: Leibovitz’s L-15 Medium + 10% FBS
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 100% Air
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% (w/v) Trypsin- 0.03 mM EDTA solution
      • Harvesting time:-
      • Seeding density:-
      • Recommended split ratio: 1:3 to 1:8
      • Medium Renewal: Every 2 to 3 days
    • Cryopreservation:
      • Freezing medium:
        • Complete growth medium + 5% (v/v) DMSO
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • Cancer research
    • 3D cell culture

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