Murata-Kamiya et al., 2007 showed that cagA expression in MKN-28 cell line results in upregulation of p21WAF1/Cip1 using western blotting.
Cell line: MKN-28 stably expressing inducible CagA under the control of tet-off system (depletion of doxycycline induces expression of CagA)
Methods: Western blotting
Murata-Kamiya et al., 2007. Helicobacter pylori CagA interacts with E-cadherin and deregulates the beta-catenin signal that promotes intestinal transdifferentiation in gastric epithelial cells. Oncogene. 26(32), 4617-4626. PMID-17237808; Full Text Links: Nature