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Laboratory Notes

 Posted in Lab Notes, Topic

Topic: Plasmid

 admin  March 19, 2023  Leave a Comment on Topic: Plasmid
  • Plasmid 
  • Plasmidome
  • Plasmid: Timeline of Key Events  
    • Discovery of the Transforming Principle: Frederick Griffith’s Pioneering Work 
    • Genetic Exchange in Bacteria: Lederberg and Tatum’s Pioneering Work
  • Natural Hosts of Plasmids Across Life Domains   
  • Plasmid Copy Number  
  • Factors Influencing Plasmid Host-Range Specificity in Bacteria
  • Plasmid Types Based on Plasmid-Encoded Traits 
    • Conjugative Plasmids   
    • Maintenance Plasmids   
    • Bacteriocin Plasmids 
    • Metabolic Plasmids 
    • Virulence Plasmids 
    • Resistance (R) Plasmids
  • Plasmid DNA Vs Chromosomal DNA 
  • Linear Plasmid  
  • Plasmid: Geometry and Conformation
  • Plasmid vs Virus
  • Plasmid pSRQ700   
  • pEC156
  • Ti Plasmid    
  • 2µ Plasmid 
  • Restriction enzyme-free Cloning
  • Restriction Digestion Analysis of Plasmid DNA
  • Protocol: Double Digestion of Plasmid DNA with FastDigest EcoRV and HindIII Restriction Enzymes
  • Protocol: Preparing T-vector using Terminal Transferase and ddTTP
  • Vacuum Manifold for Plasmid Purification Columns
  • Protocol: Size Determination of Plasmid DNA Using Agarose Gel Electrophoresis   
  • Chapter: Plasmid Isolation
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