35-mm Dishes for Cell Culture

The 35-mm dishes are small rounded dishes. The diameter of these dishes is 35 mm. However, the diameter (excluding the wall width) for cell growth is 24 mm which corresponds to a 9 cm2 area. They are often used for specific experiments which involve transfection/treatment and collecting lysate for protein or RNA analysis.

Available area for cell growth9 cm2NA
Volume of trypsin (or dissociating agent)0.7 ml
0.5 – 1 ml
Volume of culture medium1 ml0.8 – 1.5 ml
Cell number at the time of seeding *0.18 x 106 cells Ɨ0.16 – 0.32 x 106 cells
Cell number at 100% confluency *1.15 x 106 cells 1.1 – 1.3 x 106 cells # 
* for the HeLa cell line
Ɨ Correspond to seeding density 2 x 104 cells/cm2 (Ref)
# Overconfluent culture after this stage

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