The 35-mm dishes are small rounded dishes. The diameter of these dishes is 35 mm. However, the diameter (excluding the wall width) for cell growth is 24 mm which corresponds to a 9 cm2 area. They are often used for specific experiments which involve transfection/treatment and collecting lysate for protein or RNA analysis.
| Specifications | Optimum | Range |
| Available area for cell growth | 9 cm2 | NA |
| Volume of trypsin (or dissociating agent) | 0.7 ml | 0.5 – 1 ml |
| Volume of culture medium | 1 ml | 0.8 – 1.5 ml |
| Cell number at the time of seeding * | 0.18 x 106 cells Ɨ | 0.16 – 0.32 x 106 cells |
| Cell number at 100% confluency * | 1.15 x 106 cells | 1.1 – 1.3 x 106 cells # |
| * for the HeLa cell line Ɨ Correspond to seeding density 2 x 104 cells/cm2 (Ref) # Overconfluent culture after this stage | ||
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