Category: Lab Notes

Tris base (C4H11NO3) is an organic compound of four elements: Carbon, Hydrogen, Nitrogen, and Oxygen. The molecular weight of Tris base is 121.13574 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

The normality of Hydrochloric acid is equal to the molarity of Hydrochloric acid which means that a solution of 1M is also a 1N solution of hydrochloric acid.

Bicine (C6H13NO4) is an organic compound of four elements: Carbon, Hydrogen, Nitrogen, and Oxygen. The molecular weight of Bicine is 163.17254 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

A 100 – 200 ng plasmid DNA is sufficient to analyze by restriction digestion. Here we describe a procedure to double digest plasmid DNA with FastDigest EcoRV and HindIII restriction enzymes (from ThermoFisher Scientific). This procedure can be used to check the presence of a DNA fragment cloned in a vector. Double digestion will release the fragment of the appropriate size. 

Pyridine (C5H5N) is an organic compound of three elements: Carbon, Hydrogen, and Nitrogen. The molecular weight of Pyridine is 79.10024 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Sodium molybdate (Na2MoO4) is an inorganic compound of three elements: Sodium, Molybdenum, and Oxygen. The molecular weight of Sodium molybdate is 205.92713856 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Sodium sulfate (Na2SO4) is an inorganic compound of three elements: Sodium, Sulfur, and Oxygen. The molecular weight of Sodium sulfate is 142.04313856 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.