Category: RNA Analysis

Protocol – RNA Isolation from Tissue Using Trizol

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Overview: ♦ Tissues are composed of multiple cell layers that are tightly glued together by extracellular matrix. ♦ Solubilizing all…

Protocol – RNA Isolation from Suspension Cell Culture Using Trizol

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Overview: Cell lines are a very useful tool for life science research. Isolation of total RNA from cell lines is…

Protocol – RNA Isolation from Escherichia coli Using Trizol

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Overview: Escherichia coli (E. coli) is one of the well characterized bacteria and is extensively used in both applied and…

Protocol – RNA Isolation from Adherent Monolayer Cell Culture Using Trizol

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Overview: Cell lines are very useful tools for life science research. Isolation of total RNA from cell lines is often…

List of Monophasic Solution for RNA Extraction

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TRIzol ™  Reagent (Invitrogen)  Supplied by ThermoFisher Scientific  TRI Reagent®  Supplied by MRC, Sigma Aldrich, Zymoresearch RNAzol® (Tel-Test) RNA Stat-60…

Isolation of RNA Using Trizol

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Trizol is a very popular reagent among researchers for RNA isolation due to the simplicity and reliability of the isolation…

Protocol – Preparing RNA free of DNA Using DNase I Digestion

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Overview DNase I is an endonuclease that selectively cleaves phosphodiester bonds of both single- and double-stranded DNA without cleaving RNA….

Preparing RNA free of DNA

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DNA-free RNA preparations are absolutely required for applications like gene expression analysis using PCR-based methods (e.g., real-time PCR). No RNA…