Tag: agarose gel electrophoresis

Note: Agarose (for protein analysis) can be purchased from the following suppliers. You must visit the supplier’s product page to…

Note: Agarose (for cell culture) can be purchased from the following suppliers. You must visit the supplier’s product page to…

Note: Low-gelling Agarose can be purchased from the following suppliers. You must visit the supplier’s product page to order and…

Note: Agarose tablets can be purchased from the following suppliers. You must visit the supplier’s product page to order and…

Note: High-gelling Agarose can be purchased from the following suppliers. You must visit the supplier’s product page to order and…

Note: Low-melting agarose can be purchased from the following suppliers. You must visit the supplier’s product page to order and…

Note: Suppliers: Agarose (for preparative gel electrophoresis) can be purchased from the following suppliers. You must visit the supplier’s product page…

Note: Agarose (for high resolution of large DNA fragments) can be purchased from the following suppliers. You must visit the supplier’s…

Note: Agarose (for High Resolution of Small DNA Fragments) can be purchased from the following suppliers. You must visit the supplier’s…

Agarose concentration in a gel is expressed in percentage of agarose to the volume of the buffer (w/v). For example,…

Usually agarose gel electrophoresis is performed at constant low voltage (5 – 10 V/cm). High voltage results in excessive heat…

GelRed is a DNA staining dye and can be used to visualize DNA embedded in agarose gel. Both precast (GelRed…

Agarose gel electrophoresis is extensively used for the analysis of nucleic acid. Observations made by running DNA or RNA samples on agarose gel are very helpful to determine quantity and quality of nucleic acids. These powerful features make agarose gel electrophoresis an important essential step in many well known techniques such as northern and southern blotting, RFLP, RAPD, PCR, molecular cloning etc. Since agarose gel allows the elution of biomolecules, it can also be used for the purification of nucleic acids and large protein molecules and complexes. In addition, it can also be used to analyse biological processes such as apoptosis.

Agarose gel, a porous matrix, acts as a sieve through which molecules can move. The electric field is the force that forces molecules to move through an agarose gel matrix. The molecules must have a net charge, positive or negative, in order to move in an electric field. A neutral molecule will not move in an electric field.

Bromophenol blue and Xylene cyanol are the two most commonly used tracking dyes for the analysis of DNA on agarose gel electrophoresis. These negatively charged dyes not only help in monitoring the progress of agarose gel electrophoresis but also allow easy monitoring of sample loading process onto the wells of agarose gel. Their position in relation to DNA fragments is an important information that helps to determine how far a gel must be run without accidentally letting the DNA fragments of interest run out of the gel and at the same time ensuring good resolution among different size DNA fragments.