Tag: agarose

Suppliers: Agarose (for Protein Analysis)

Note: Agarose (for protein analysis) can be purchased from the following suppliers. You must visit the supplier’s product page to…

Suppliers: Agarose (for Cell Culture)

Note: Agarose (for cell culture) can be purchased from the following suppliers. You must visit the supplier’s product page to…

Suppliers: Low-Gelling Agarose

Note: Low-gelling Agarose can be purchased from the following suppliers. You must visit the supplier’s product page to order and…

Suppliers: Agarose Tablet

Note: Agarose tablets can be purchased from the following suppliers. You must visit the supplier’s product page to order and…

Suppliers: High-Gelling Agarose

Note: High-gelling Agarose can be purchased from the following suppliers. You must visit the supplier’s product page to order and…

Suppliers: Low-Melting Agarose

Note: Low-melting agarose can be purchased from the following suppliers. You must visit the supplier’s product page to order and…

Suppliers: Agarose (for Preparative Gel Electrophoresis)

Note: Suppliers: Agarose (for preparative gel electrophoresis) can be purchased from the following suppliers. You must visit the supplier’s product page…

Suppliers: Agarose (for High Resolution of Large DNA Fragments)

Note: Agarose (for high resolution of large DNA fragments) can be purchased from the following suppliers. You must visit the supplier’s…

Suppliers: Agarose (for High Resolution of Small DNA Fragments)

Note: Agarose (for High Resolution of Small DNA Fragments) can be purchased from the following suppliers. You must visit the supplier’s…

Tips to Reduce Run-time of Agarose Gel Electrophoresis

Usually agarose gel electrophoresis is performed at constant low voltage (5 – 10 V/cm). High voltage results in excessive heat…

Electroendosmosis in Agarose Gel Electrophoresis

In agarose gel electrophoresis, electroendosmosis (EEO) can be defined as ‘the active water transport towards cathode (negative electrode) from anode…

Recommended Agarose Gel Percentage for Efficient Separation of DNA Fragments

Agarose Gel is a porous matrix that acts as a sieve through which negatively charged linear DNA fragments migrate under an…

Applications of Agarose Gel Electrophoresis

Agarose gel electrophoresis is extensively used for the analysis of nucleic acid. Observations made by running DNA or RNA samples on agarose gel are very helpful to determine quantity and quality of nucleic acids. These powerful features make agarose gel electrophoresis an important essential step in many well known techniques such as northern and southern blotting, RFLP, RAPD, PCR, molecular cloning etc. Since agarose gel allows the elution of biomolecules, it can also be used for the purification of nucleic acids and large protein molecules and complexes. In addition, it can also be used to analyse biological processes such as apoptosis.

Agarose Gel Electrophoresis of DNA

DNA agarose gel electrophoresis is an analytical technique of analyzing and separating DNA fragments based on their size. Agarose gel…

Agarose Gel Electrophoresis

Agarose gel, a porous matrix, acts as a sieve through which molecules can move. The electric field is the force that forces molecules to move through an agarose gel matrix. The molecules must have a net charge, positive or negative, in order to move in an electric field. A neutral molecule will not move in an electric field.