Tag: plasmid isolation

Protocol – Preparing Liquid Culture of E. coli for Plasmid Miniprep

 admin    0 Comments on Protocol – Preparing Liquid Culture of E. coli for Plasmid Miniprep

Overview: A small-scale plasmid isolation procedure, the miniprep, yields a sufficient amount of plasmid for the screening of clones and…

Preparation of Glucose-containing Resuspension Buffer for Plasmid Isolation by Alkaline Lysis Method

 admin    1 Comment on Preparation of Glucose-containing Resuspension Buffer for Plasmid Isolation by Alkaline Lysis Method

Overview: Resuspension buffer is used to resuspend the harvested bacterial cells from the culture during plasmid isolation by the alkaline…

Preparation of Neutralization Solution (Solution III) for Plasmid Isolation by Alkaline Lysis Method

 admin    1 Comment on Preparation of Neutralization Solution (Solution III) for Plasmid Isolation by Alkaline Lysis Method

Overview The neutralization solution (solution III) is used for the isolation of plasmid DNA by the alkaline lysis method. The…

Preparation of Lysis Solution (Solution II) for Isolation of Plasmid by Alkaline Lysis Method

 admin    1 Comment on Preparation of Lysis Solution (Solution II) for Isolation of Plasmid by Alkaline Lysis Method

Overview Lysis solution (solution II) is used for the isolation of plasmid DNA by the alkaline lysis method. The plasmid-containing…