Tag: ampicillin
Ampicillin
Ampicillin belongs to a group of beta-lactam antibiotics. It is classified as critically important human medicine by WHO. Ampicillin is one of the synthetic derivatives of penicillin. It was generated by adding an amino group side chain on penicillin. Ampicillin inhibits the synthesis of the bacterial cell wall by stopping the peptidoglycan cross-linking. It is effective against both gram-positive and gram-negative bacteria.
Preparation of Stock Solution of Ampicillin (50 mg/ml)
Ampicillin is one of the most extensively used antibiotics as a selection marker in molecular biology and bacteriology. Ampicillin is available as Ampicillin anhydrous, Ampicillin trihydrate and Ampicillin sodium salt. Ampicillin sodium salt is often used to prepare stock solution as it has better solubility in water (>50 mg/ml in water). A 50 mg/ml stock solution of ampicillin can be prepared by dissolving 500 mg ampicillin sodium salt in water to a final volume of 10 ml.