Category: Electrophoresis

Temperature change during electrophoresis is an important parameter to consider when optimizing already existing reagents or conditions or testing new…

Agarose concentration in a gel is expressed in percentage of agarose to the volume of the buffer (w/v). For example,…

Changes in electrophoresis buffer pH is an important parameter to consider when optimizing already existing reagents or conditions or testing…

The TBE (Tris-Borate-EDTA) electrophoresis buffer contains Tris base, Boric acid, EDTA and has pH 8.3. Usually the stock TBE electrophoresis…

Usually agarose gel electrophoresis is performed at constant low voltage (5 – 10 V/cm). High voltage results in excessive heat…

Ethidium Bromide is a DNA staining dye and can be used to visualize DNA in agarose gel. Both precast (Ethidium…

There are several dyes (Bromophenol blue, Bromocresol green, Orange G, Xylene cyanol FF) that can be used for the preparation…

GelRed is a DNA staining dye and can be used to visualize DNA embedded in agarose gel. Both precast (GelRed…

Overview DNA loading dye containing only one tracking dye Bromophenol blue (BPB) can be used to analyze larger DNA fragments…

In agarose gel electrophoresis, electroendosmosis (EEO) can be defined as ‘the active water transport towards cathode (negative electrode) from anode…

OVERVIEW Plasmids are circular DNA. Their size can not be determined in their native state as the migration of circular…

OVERVIEW Approximate size of DNA fragments can easily be determined by agarose gel electrophoresis. This analysis is quite useful to…

Agarose Gel is a porous matrix that acts as a sieve through which negatively charged linear DNA fragments migrate under an…

Overview DNA sample is mixed with DNA loading dye prior to loading into the wells of agarose gel.  DNA loading…

A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.