Tag: cryopreservation
Protocol – Thawing and Revival of Cryopreserved Cells
This protocol describes the revival process of cryopreserved adherent cells. The revival process involves rapid thawing of cryopreserved cells followed by removal of freezing medium which contains cryoprotectant, in this case, DMSO. The freezing medium can be removed immediately just after thawing the cells by centrifugation. Alternatively, cells are allowed to adhere to the culture dish followed by washing and addition of fresh growth medium.
Cryopreservation of Cells
Cryopreservation is an efficient way to preserve cells at ultra-low temperatures (below -135°C) which stops all physiological processes and biological aging. It is a routinely used technique in all cell culture laboratories.
Preparation of Freezing Medium Containing DMSO and Serum
Overview The freezing medium is used to preserve cell lines at ultra-low temperatures. This method of preserving cell lines is…
Protocol – Cryopreservation of Adherent Cell Culture
Overview Cryopreservation of adherent cell cultures requires detaching the cells from the substratum, typically through trypsinization. The detached cells are…