Tag: Cryopreservation

This protocol describes the revival process of cryopreserved adherent cells. The revival process involves rapid thawing of cryopreserved cells followed by removal of freezing medium which contains cryoprotectant, in this case, DMSO. The freezing medium can be removed immediately just after thawing the cells by centrifugation. Alternatively, cells are allowed to adhere to the culture dish followed by washing and addition of fresh growth medium.

Overview The freezing medium is used to preserve cell lines at ultra-low temperatures. This method of preserving cell lines is…

Overview Cryopreservation is an efficient way of preserving cell culture at ultra-low temperatures below -135°C. Preserved cells can be revived…