Tag: cryopreservation

Protocol – Thawing and Revival of Cryopreserved Cells

This protocol describes the revival process of cryopreserved adherent cells. The revival process involves rapid thawing of cryopreserved cells followed by removal of freezing medium which contains cryoprotectant, in this case, DMSO. The freezing medium can be removed immediately just after thawing the cells by centrifugation. Alternatively, cells are allowed to adhere to the culture dish followed by washing and addition of fresh growth medium.

Cryopreservation of Cells

Cryopreservation is an efficient way to preserve cells at ultra-low temperatures (below -135°C) which stops all physiological processes and biological aging. It is a routinely used technique in all cell culture laboratories.

Preparation of Freezing Medium Containing DMSO and Serum

Overview The freezing medium is used to preserve cell lines at ultra-low temperatures. This method of preserving cell lines is…

Protocol – Cryopreservation of Adherent Cell Culture

Overview Cryopreservation (Cryo: icy cold or frost; Preservation: Storage) is an efficient way of preserving cell culture at ultra-low temperatures…