Category: Bacterial Growth Media

Preparation of Lambda-agar medium and plates

Lambda-agar is nothing but a lambda broth which is solidified by agar. Agar is a solidifying agent. It melts at 85°C but melton agar stays liquid at a temperature > 40°C (this property is called hysteresis, different temperatures for melting and solidifying). This allows us to add heat-labile medium supplements such as antibiotics and vitamins and prepare solid medium. Lambda-agar solid medium is prepared by dissolving 15 g agar / liter of Lambda broth.

Preparation of TNT Medium

OVERVIEW TNT medium is a moderately rich medium. It contains tryptone, sodium chloride (NaCl) and thiamine. Tryptone provides basic nutrients…

Preparation of M9 Minimal Medium

OVERVIEW M9 minimal medium is a chemically-defined minimal growth medium, used to cultivate Escherichia coli. It is prepared by supplementing…

Preparation of SOC (Super Optimal broth with Catabolite repression) Medium

SOC (Super Optimal broth with Catabolite repression) medium is a nutrient-rich microbial growth medium, formulated by Douglas Hanahan in 1983 (Hanahan, 1983). SOC medium is prepared without Mg++ and Glucose by dissolving 20 g bacto-tryptone, 5 g bacto-yeast extract, 2 ml of 5M sodium chloride, and 2.5 ml of 1M KCl in water to a final volume of ≈ 1000 ml (960 ml). Magnesium salts (i.e., MgSO4 and MgCl2, 10 ml each of 1M MgSO4 and 1M MgCl2) and Glucose (10 ml of 1 M Glucose) are added to the medium after autoclaving to prevent any chemical reactions and precipitation of the medium components, and degradation of Glucose.

LB Luria Broth

Preparation of LB Luria Broth

LB is a rich growth medium, commonly used to grow E. coli. LB Luria Broth contains 1% Bacto-tryptone, 0.5% Bacto-yeast extract and 0.05% Sodium chloride. A 1000 ml LB Luria Broth is prepared by dissolving 10 g Bacto-tryptone, 5 g Bacto-yeast extract and 0.5 g sodium chloride in deionized water to a final volume of 1000 ml. After adjusting the pH 7.0 with 5N NaOH medium is autoclaved and stored in a cold room.

Preparation of LB Lennox Broth

LB medium is a rich growth medium, commonly used to grow E. coli. LB, Lennox Broth contains 1% Bacto-tryptone, 0.5% Bacto-yeast extract and 0.5% Sodium chloride. A 1000 ml LB, Lennox Broth is prepared by dissolving 10 g Bacto-tryptone, 5 g Bacto-yeast extract and 5 g sodium chloride in deionized water to a final volume of 1000 ml. After adjusting the pH 7.0 with 5N NaOH medium is autoclaved and stored in a cold room.

Preparation of Lambda Broth

OVERVIEW Lambda Broth is a moderately rich medium. It is useful in the propagation of λ Phage. It contains tryptone…