Category: Cell Culture

T-225 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a…

T-175 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a…

T-75 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a…

T-25 flasks are available from different suppliers. They are suitable for routing maintenance of a cell line. They are available…

OVERVIEW AGS cells grow as an adherent monolayer. Therefore, detaching cells from the substrate using specific means is required for…

Various culture media have been used to maintain the AGS cell line in culture and for specific experimental procedures. A 10% fetal bovine serum (FBS)-supplemented RPMI medium is the most popular medium used by many researchers to maintain AGS cells in culture. Some have also used 10% FBS-supplemented DMEM. ATCC suggests Ham’s F12K Medium supplemented with 10% FBS. A list of base media used for AGS cell line, experiment-specific supplements, culture conditions, purpose, and references are given in the table below.

Overview: ♦ Tissues are composed of multiple cell layers that are tightly glued together by extracellular matrix. ♦ Solubilizing all…

Overview: Cell lines are a very useful tool for life science research. Isolation of total RNA from cell lines is…

Culture vessel types Surface area Culture flask T-25 25 cm2 T-75 75 cm2 T-175 175 cm2 Culture dish 35 mm…

Overview Preparation of metaphase chromosome spread is an important technique in cytogenetics. Metaphase chromosome spread is used for karyotyping including…

The serum is commercially supplied in different sizes with 500 ml being the most common size. Since repeated freezing and…

Laminar flow hood (Class II) provides a sterile environment for handling cell culture. To make the laminar flow hood ready…

Overview Heat inactivation of serum refers to the process which involves treatment of serum at a higher temperature to inactivate…

Complete replacement of culture medium from suspension culture involves cell harvesting by centrifugation and resuspension of the cell pellet in fresh growth medium. This method is ideally the best way to feed suspension culture and is often used when a. you observe dead or sick cells/suspended particles in culture; b. medium color (phenol red-containing medium) has turned yellow very quickly; c. you can not afford cell loss. Complete replacement of culture medium is ideally the best way to feed suspension culture. Since this procedure takes time and involves an additional centrifugation step to harvest cells, often researchers partially replace the medium that takes just a few minutes. However, partial medium replacement is associated with the loss of cells from the culture.