Category: Cell Culture

T-225 Flasks for Cell Culture

T-225 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a…

T-175 flasks for cell culture

T-175 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a…

T-75 Flasks for Cell Culture

T-75 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a…

T-25 Flasks for Cell Culture

T-25 flasks are available from different suppliers. They are suitable for routing maintenance of a cell line. They are available…

AGS Cell Line: Subculturing Procedure

OVERVIEW AGS cells grow as an adherent monolayer. Therefore, detaching cells from the substrate using specific means is required for…

AGS Cell Line: Culture Media

Various culture media have been used to maintain the AGS cell line in culture and for specific experimental procedures. A…

Protocol – RNA Isolation from Tissue Using Trizol

Overview: ♦ Tissues are composed of multiple cell layers that are tightly glued together by extracellular matrix. ♦ Solubilizing all…

Protocol – RNA Isolation from Suspension Cell Culture Using Trizol

Overview: Cell lines are a very useful tool for life science research. Isolation of total RNA from cell lines is…

Commonly Used Cell Culture Vessels

Culture vessel types Surface area Culture flask T-25 25 cm2 T-75 75 cm2 T-175 175 cm2 Culture dish 35 mm…

Preparation of Metaphase Chromosome Spreads from Adherent Cell Line

Overview Preparation of metaphase chromosome spread is an important technique in cytogenetics. Metaphase chromosome spread is used for karyotyping including…

Handling and Aliquoting Frozen Stock of Serum

The serum is commercially supplied in different sizes with 500 ml being the most common size. Since repeated freezing and…

Preparing Laminar Flow Hood for Cell Culture Work

Laminar flow hood (Class II) provides a sterile environment for handling cell culture. To make the laminar flow hood ready…

Protocol – Heat Inactivation of Serum

Overview Heat inactivation of serum refers to the process which involves treatment of serum at a higher temperature to inactivate…

Protocol – Feeding Suspension Culture (Complete Replacement of Medium)

Complete replacement of culture medium from suspension culture involves cell harvesting by centrifugation and resuspension of the cell pellet in fresh growth medium. This method is ideally the best way to feed suspension culture and is often used when a. you observe dead or sick cells/suspended particles in culture; b. medium color (phenol red-containing medium) has turned yellow very quickly; c. you can not afford cell loss. Complete replacement of culture medium is ideally the best way to feed suspension culture. Since this procedure takes time and involves an additional centrifugation step to harvest cells, often researchers partially replace the medium that takes just a few minutes. However, partial medium replacement is associated with the loss of cells from the culture.