Category: Cell Culture

Passage number refers to the number of times cells have been subcultured or passaged  i.e., upon confluence, cells are transferred…

Mycoplasma contaminated culture can either be discarded or be cured. The best approach is to discard the culture because: It…

Several mycoplasma species from human, porcine and bovine origin have been detected in cell culture. The following eight species account…

Mycoplasma contamination is a common and challenging problem in cell culture laboratories, but the following measures can substantially reduce the…

Mycoplasma contamination is a prevalent issue in cell culture laboratories, posing significant challenges. However, a comprehensive understanding of mycoplasma contamination…

The following species of mycoplasma are most frequently detected in cell culture: Mycoplasma pirum Mycoplasma salivarium Mycoplasma orale Mycoplasma fermentans…

Detection of mycoplasma is not as easy as detection of bacterial and yeast contamination. Mycoplasma contaminated cultures do not show…

Mycoplasma contamination is still a challenging problem in most cell culture laboratories. Due to their small size, they escape microscopic…

T-225 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a…

T-175 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a…

T-75 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a…

T-25 flasks are available from different suppliers. They are suitable for routing maintenance of a cell line. They are available…

OVERVIEW AGS cells grow as an adherent monolayer. Therefore, detaching cells from the substrate using specific means is required for…

Various culture media have been used to maintain the AGS cell line in culture and for specific experimental procedures. A 10% fetal bovine serum (FBS)-supplemented RPMI medium is the most popular medium used by many researchers to maintain AGS cells in culture. Some have also used 10% FBS-supplemented DMEM. ATCC suggests Ham’s F12K Medium supplemented with 10% FBS. A list of base media used for AGS cell line, experiment-specific supplements, culture conditions, purpose, and references are given in the table below.

Overview: ♦ Tissues are composed of multiple cell layers that are tightly glued together by extracellular matrix. ♦ Solubilizing all…