Category: Reagents

♦ A homogeneous mixture of two or more non-reactive substances is called a solution. A solution is prepared by dissolving…

A stock solution can be defined as a highly concentrated solution, mostly prepared as a 10x concentrated solution, which can be diluted to prepare a working solution. Stock solutions can also be used as a component to prepare complex solutions containing many ingredients. For example, a stock solution of EDTA (0.5 M EDTA solution) can be used to prepare many solutions which contain EDTA e.g., TAE, TBE, Tris-EDTA, Trypsin-EDTA etc.

Metal Ion-EDTA complex Stability constant (log kf) Al3+–EDTA complex 16.4 Ca2+–EDTA complex 10.65 Cd2+–EDTA complex 16.5 Co2+–EDTA complex 16.45 Co3+–EDTA…

Overview: Ethidium bromide (EtBr) is a commonly used fluorescent stain to visualize nucleic acid especially DNA in agarose gels. Ethidium…

Acids / bases Molecular formula Molecular weight Strength of Concentrated reagent as supplied commercially Specific gravity (g/ml) at 25 °C…

Overview Chloramphenicol is a broad-spectrum antibiotic that is effective against both gram-positive and gram-negative bacteria. It is a bacteriostatic agent….

Ampicillin is one of the most extensively used antibiotics as a selection marker in molecular biology and bacteriology. Ampicillin is available as Ampicillin anhydrous, Ampicillin trihydrate and Ampicillin sodium salt. Ampicillin sodium salt is often used to prepare stock solution as it has better solubility in water (>50 mg/ml in water). A 50 mg/ml stock solution of ampicillin can be prepared by dissolving 500 mg ampicillin sodium salt in water to a final volume of 10 ml.

Tracking dyes are colored dyes, which are added to the loading dye/buffer. Loading dye/buffers are used to prepare samples for…

Overview Sodium dodecyl sulfate (SDS,  CH3(CH2)11OSO3Na, molecular weight 288.38 g/mole, CAS Number 151-21-3) is an anionic surfactant (detergent) and is…

The 2 x YT medium, also known as a 2 x TY medium, is a rich medium. It is recommended for the growth and propagation of E. coli infected with the single strand filamentous bacteriophage M13. It can be prepared by dissolving 16 g tryptone, 10 g yeast extract, and 5 g Sodium chloride (NaCl) in water to a final volume of 1000 ml.

Overview DTT (DTT, C4H10O2S2, molecular weight 154.25 g/mole) is also known as Cleland’s reagent. DTT is commonly used for the…

EDTA is a polyaminocarboxylic acid. Its conjugate base is  ethylenediaminetetraacetate. It is a colorless compound that was first synthesized by…

Agarose is a useful matrix for a number of analytical and preparative techniques including gel electrophoresis, chromatography, and support matrix to immobilize enzymes and cells. Agarose is available in a variety of forms, which differ in physical properties. The two most common agarose are standard agarose and low melting agarose. Standard agarose is most commonly used for analysis of DNA and RNA. Low melting agarose is often used for preparative purposes such as elution of DNA fragments and complexes.

Overview EDTA (Ethylenediaminetetraacetic acid) is a chelating agent. Due to its ability to sequester metal ions such as Mn2+, Ca2+,…

10 ml of 6x DNA loading dye containing Bromophenol blue, Xylene cyanol FF, and Ficoll 400 is prepared by dissolving 25 mg bromophenol blue, 25 mg xylene cyanol FF and 1.5 g Ficoll 400 in water to a final volume of 10 ml. The final solution (6x DNA loading dye) will contain 0.25% (w/v) bromophenol blue, 0.25% (w/v) xylene cyanol FF, and 15% (w/v) Ficoll 400.