Overview
- Chloramphenicol is a broad-spectrum antibiotic that is effective against both gram-positive and gram-negative bacteria.
- It is a bacteriostatic agent.
- It inhibits bacterial protein synthesis by irreversibly binding to 50S ribosomal subunit and inhibiting peptidyl transferase catalytic function.
Requirement
Reagents
> Chloramphenicol
> 70% ethanol
Equipment and disposables
> Measuring cylinder
> Conical flask / Beaker / 15 ml screw-capped tube
Composition
> 34 mg/ml chloramphenicol
> 70% ethanol
Objective
Preparation of 10 ml of chloramphenicol stock solution in 70% ethanol
Preparation
Step 1: To prepare 10 ml of stock solution of chloramphenicol, weigh out 340 mg of chloramphenicol. Transfer it to a 15 ml screw-capped tube. Add 9 ml of 70% ethanol. Mix until all chloramphenicol dissolves completely.
Tip:
We recommend using a 15 ml screw-capped tube with milliliter marks. Transfer all the contents in it. Add 9 ml of 70% ethanol and mix the content by rotating the tube. Once the content is mixed, make up the volume to 10 ml.
Precaution:
Do not dissolve in 10 ml of deionized / Milli-Q water. In most cases, solution volume increases when a large amount of solute dissolves in a solvent.
Step 2: Adjust the volume to 10 ml with 70% ethanol. Mix it again.
Storage
Store the solution in small aliquots (1 ml) at -20°C for a long time.
Tip:
It is recommended to store the chloramphenicol stock solution in small aliquots.
Applications
A selection marker for bacterial culture during gene cloning experiments.
