Category: Reagents

Preparation of 1N Sodium Hydroxide (NaOH) Solution by Diluting 10N Solution

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Preparation of 10N Sodium Hydroxide (NaOH)

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Preparation of 10x Tris-Glycine-SDS Running Buffer for SDS-PAGE using 20% SDS Solution

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Preparation of 0.5 M Sodium Fluoride Solution

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Preparation of Stock Solution of Sodium Orthovanadate (0.2 M Na3VO4 in Water)

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Preparation of 5M Hydrochloric Acid

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Preparation of 1N Hydrochloric Acid

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Preparation of Stock Solution of G-418 (100 mg/ml in water)

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Preparation of 50 mg/ml Gentamicin Stock Solution

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Preparation of 20% Tween 20 Solution

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Preparation of 20% Triton X-100 Solution

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Preparation 10% Sodium Azide Stock Solution

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Preparation of 10 M Sodium Hydroxide from 50% (w/w) Stock Solution

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Preparation of 1M MOPS (Free Acid) Solution

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Preparation of 6X DNA Loading Buffer (Orange G, Xylene Cyanol FF and Ficoll 400)

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A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.