Category: Reagents

The normality and molarity of the Sodium hydroxide (NaOH) solution are the same. This means the 10N solution of sodium…

OVERVIEW Tris-Glycine-SDS running buffer is commonly used to resolve proteins in SDS-polyacrylamide gel. A concentrated buffer is usually prepared that…

OVERVIEW A 0.5 M (or 500 mM) stock solution (250x concentrated) of Sodium fluoride (NaF) can be prepared by dissolving…

OVERVIEW A 0.2 M (or 200 mM) stock solution (100x concentrated) of Sodium orthovanadate (Na3VO4) can be prepared by dissolving…

OVERVIEW 5M hydrochloric acid can be prepared by diluting a concentrated 37% (w/w) stock solution of hydrochloric acid. A 37%…

Hydrochloric acid is supplied as a concentrated stock solution [37% (w/w)]. The molarity of the 37% (w/w)  solution is 12.178…

OVERVIEW G-418 (also called G418, or G 418) is a selective antibiotic that is effective against many live organisms including…

OVERVIEW Gentamicin is an aminoglycoside antibiotic. It is effective against a wide variety of aerobic bacteria including E. coli. A…

OVERVIEW Tween 20 is a non-ionic detergent and is used in many biological applications including immunochemistry and western blotting. Tween…

OVERVIEW Triton X-100 is a non-ionic, mild, non-denaturing detergent, used for solubilizing cell membrane, without denaturing proteins. It is often…

OVERVIEW Commercially, sodium azide is supplied as a white powder. It is added to a solution to prevent the growth…

OVERVIEW A 50% (w/w) Sodium Hydroxide can be purchased from several commercial suppliers. It is a clear colorless liquid and…

Overview MOPS (Free acid, C7H15NO4S, Molecular weight: 209.26) is a zwitterionic buffer, useful for several cell and molecular biology applications…

A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.

Magnesium Chloride (MgCl2) is soluble in water. Magnesium chloride is available in anhydrous as well as hydrated forms. Since anhydrous Magnesium chloride is hygroscopic (quickly absorbs water from the air), it is convenient to use hydrated forms that are easy to weigh out the right amount accurately. For this reason, Magnesium chloride hexahydrate (MgCl2.​6H2O, Molecular Weight: 203.30) is often used to prepare a 1 M Magnesium chloride solution. A 1M stock solution can be prepared by dissolving 203.3 g of MgCl2.​6H2O (Molecular Weight: 203.30) in water to a final volume of 100 ml. In the following section, we describe a procedure to prepare 100 ml of 1M MgCl2 stock solution.