- Synonyms:
- 293T/17
- HEK 293T/17
- Description:
- Parent cell line: 293 (also known as HEK-293)
- STAT1 BAX double knockout HEK-293 cell line, generated using CRISPR-Cas9 gene editing technology
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth Mode: Adherent
- Doubling time: ≈ 20h
- Morphology: Epithelial-like
- Applications:
- Adeno-associated virus (AAV) production for gene therapy applications
- Vaccine development
- Virus propagation
- Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- DMEM + 10% FBS
- DMEM + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Cell dissociation procedure: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: 80% confluence culture
- Subcultivation Ratio: 1:6 to 1:10
- Medium Renewal: 2 to 3 times per week
- Seeding densities: 1 x 104 and 4 x 104 viable cells/cm2
- Cell dissociation procedure: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Cryopreservation
- Complete Growth medium + 5% DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- https://www.atcc.org/products/crl-1573-vhg
- Horvath & Darnell, 1996. The Antiviral State Induced by Alpha Interferon and Gamma Interferon Requires Transcriptionally Active Stat1 Protein. J Virol 70: 647-650, 1996. PMID-8523587; Full-Text Links: ASM, PMC189860
- Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Fetal Bovine Serum)
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