- Synonyms:
- 293
- HEK 293
- HEK-293
- Human Embryonic Kidney-293
- Culture Type: Cell line
- Keywords: Human Embryonic Kidney Cell Line
- Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: fetus
- Species: Human (Homo sapiens)
- Tissue: Kidney; Embryo
- Year of isolation: 1973
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth mode: Adherent, Monolayer
- Doubling time: about 24-30 hours
- Life span: Infinite (continuous culture)
- Karyotype: Hypotriploid, Modal chromosome number was 64 (in 30% of cells)
- Transformed: Transformed with adenovirus 5 DNA
- Tumorigenic: Yes (forms tumors in nude mice)
- Gene Expression: Vitronectin receptor
- Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- GMO Status: Genetically Modified Organism Class 1 (GMO1)
- Complete culture medium: Eagle’s Minimum Essential Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: Humidified 37°C incubator
- Subculturing:
- Cell dissociation procedure: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Subcultivation Ratio: 1:6 to 1:10
- Medium Renewal: Every 2 to 3 days
- Seeding densities: 2 x 104 to 4 x 104 cells/cm2
- Harvesting densities: 6 x 104 and 7 x 104 cells/cm2
- Split time: Split sub-confluent cultures (80%)
- Cell dissociation procedure: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Cryopreservation
- Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Complete growth medium + 5% (v/v) DMSO
- Applications:
- 3D cell culture
- High-throughput screening
- Toxicology
- Industrial biotechnology
- Bioproduction
- Efficacy testing
- Transfection host
- Viruscide testing
- Availability
- American Type Culture Collection (ATCC): Product link
- ECACC General Cell Collection: Product link
- Sigma-Aldrich, Catalog No.: Product link
- Cell Line Service (CLS): Product link
- Bioresource Collection and Research Center (BCRC): Product link
- Images:
- Click here to see HEK-293 cell image (Source: ATCC)
- References
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