Tag: Molecular cloning

Restriction enzyme-free Cloning

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In contrast to conventional cloning strategies, Restriction enzyme-free cloning (also called Restriction-free cloning) involves no treatment of DNA fragments to…

Protocol: Preparing T-vector using Terminal Transferase and ddTTP

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T-vectors are linearized vectors that contain a single T-overhang at the 3’-end of both DNA strands. T-vectors are useful for…