Tag: molecular cloning
Restriction enzyme-free Cloning
In contrast to conventional cloning strategies, Restriction enzyme-free cloning (also called Restriction-free cloning) involves no treatment of DNA fragments to…
Protocol: Preparing T-vector using Terminal Transferase and ddTTP
T-vectors are linearized vectors that contain a single T-overhang at the 3’-end of both DNA strands. T-vectors are useful for…