Category: Lab Notes

A 37% (w/w) Hydrochloric acid (HCl) can be purchased from the following suppliers. You must visit the supplier’s product page to order and to get more details of the product.

A 6x DNA Gel loading buffer containing Orange G, Bromophenol blue, Xylene cyanol FF, EDTA and Ficoll 400 is prepared by dissolving 25 mg bromophenol blue, 25 mg xylene cyanol FF, 40 mg Orange G, 1.5 g Ficoll 400 and 1.2 ml of 0.5 M EDTA in 10 mM Tris.Cl, pH 7.6 to a final volume of 10 ml. The final solution (6x DNA loading dye) will contain 0.25% (w/v) bromophenol blue, 0.25% (w/v) xylene cyanol FF, 15% (w/v) Ficoll 400, 60 mM EDTA and 10 mM Tris.