Cell Line: FTC-236

OrganismHuman (Homo sapiens), 42 years old Male
Source/Tissue of origin
Thyroid, Neck lymph node metastasis, Follicular thyroid carcinoma
Cell typeEndothelial
Growth modeAdherent, monolayer
Doubling time
Life spanInfinite, continuous culture
Tumorigenic Tumorigenic in Balb/c mice
Drug resistance 
Biosafety Level2 (phe-culturecollections) (May differ in your country, follow the guidelines of your institute/country)
Gene ExpressionY-chromosome is lost
Related cell lines 

Cell line isolation information

The cell line FTC-236 was established from a neck lymph node metastasis of a follicular thyroid carcinoma from a 42-year-old male.

Recommended propagation method

Culture medium: DMEM : Ham’s F12 (1:1) + 10 µg/ml Insulin + 0.01U/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:6
Split frequency:
Harvesting time: Subconfluent culture (≈80%)
Split Method: Trypsin/EDTA
Seeding density: 20,000 – 40,000 cells/ cm2

Recommended Cryopreservation Method

Freeze medium: 40% complete culture medium + 50% FBS  + 10% DMSO

Storage temperature: Liquid nitrogen vapor phase

Cell density:



  • Demeure et al., 1992. Invasion by cultured human follicular thyroid cancer correlates with increased beta 1 integrins and production of proteases. World J Surg. 16(4), 770-776. PMID-1384245
  • Hölting et al., 1994. Transforming growth factor-beta 1 is a negative regulator for differentiated thyroid cancer: studies of growth, migration, invasion, and adhesion of cultured follicular and papillary thyroid cancer cell lines. J Clin Endocrinol Metab. 79(3), 806-813. PMID-8077365; Full Text Link: academic.oup

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