| OVERVIEW | |
| Organism | Human (Homo sapiens), 42 years old Male |
| Source/Tissue of origin | Thyroid, Neck lymph node metastasis, Follicular thyroid carcinoma |
| Morphology | |
| Cell type | Endothelial |
| Growth mode | Adherent, monolayer |
| Doubling time | |
| Life span | Infinite, continuous culture |
| Karyotype | |
| Transformed | |
| Tumorigenic | Tumorigenic in Balb/c mice |
| Drug resistance | |
| Biosafety Level | 2 (phe-culturecollections) (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | Y-chromosome is lost |
| Products | |
| Applications | |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line FTC-236 was established from a neck lymph node metastasis of a follicular thyroid carcinoma from a 42-year-old male.
Recommended propagation method
Culture medium: DMEM : Ham’s F12 (1:1) + 10 µg/ml Insulin + 0.01U/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:6
Split frequency:
Harvesting time: Subconfluent culture (≈80%)
Split Method: Trypsin/EDTA
Seeding density: 20,000 – 40,000 cells/ cm2
Recommended Cryopreservation Method
Freeze medium: 40% complete culture medium + 50% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Cell density:
AVAILABILITY
- Public Health England : Product link
- Cell Bank Australia: Product link
REFERENCES
- Demeure et al., 1992. Invasion by cultured human follicular thyroid cancer correlates with increased beta 1 integrins and production of proteases. World J Surg. 16(4), 770-776. PMID-1384245
- Hölting et al., 1994. Transforming growth factor-beta 1 is a negative regulator for differentiated thyroid cancer: studies of growth, migration, invasion, and adhesion of cultured follicular and papillary thyroid cancer cell lines. J Clin Endocrinol Metab. 79(3), 806-813. PMID-8077365; Full Text Link: academic.oup
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