|Organism||Murine (mouse) (Mus musculus), DBA/2|
|Source/Tissue of origin||Mouse mastocytoma tumor|
|Morphology||Single, round cells|
|Cell type||Mast cell|
|Growth mode||Suspension with some adherent cells|
|Doubling time||18-22 hours|
|Life span||Infinite, continuous culture|
|Biosafety Level||1 (dsmz) (May differ in your country, follow the guidelines of your institute/country)|
|Applications|| 3D cell culture, transfection host, used as target cells for cytotoxic T cell assays|
|Related cell lines|
Cell line isolation information
The cell line p-815 was established from the mastocytoma tumor of a DBA/2 mouse treated with methylcolanthrene.
Recommended propagation method
90% RPMI 1640 + 10% h.i. FBS (dsmz)
90% DMEM + 10% FBS (atcc)
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Medium Renewal: Every 2 to 3 days
Split ratio: 1:4 to 1:8
Split frequency: every 3-4 days
Harvesting time: > 1 x 106 cells/ml
Split Method: Dilution
Seeding density: ≈2 x 105 cells/ml
Recommended Cryopreservation Method
Freeze medium: 70% complete medium + 20% FBS * + 10% DMSO
* One can increase the percentage of FBS upto 50%
Storage temperature: Liquid nitrogen vapor phase
- DSMZ: Product link
- ATCC: Product link
- Ralph et al., 1974. Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD. Biochem Biophys Res Commun. 61(4), 1268-1275. PMID-4616699; Full-Text Link: sciencedirect
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