| OVERVIEW | |
| Organism | Murine (mouse) (Mus musculus), DBA/2 |
| Source/Tissue of origin | Mouse mastocytoma tumor |
| Morphology | Single, round cells |
| Cell type | Mast cell |
| Growth mode | Suspension with some adherent cells |
| Doubling time | 18-22 hours |
| Life span | Infinite, continuous culture |
| Karyotype | |
| Transformed | |
| Tumorigenic | |
| Drug resistance | |
| Biosafety Level | 1 (dsmz) (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | Lysozyme |
| Products | |
| Applications | 3D cell culture, transfection host, used as target cells for cytotoxic T cell assays |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line p-815 was established from the mastocytoma tumor of a DBA/2 mouse treated with methylcolanthrene.
Recommended propagation method
Culture medium:
90% RPMI 1640 + 10% h.i. FBS (dsmz)
90% DMEM + 10% FBS (atcc)
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Medium Renewal: Every 2 to 3 days
Split ratio: 1:4 to 1:8
Split frequency: every 3-4 days
Harvesting time: > 1 x 106 cells/ml
Split Method: Dilution
Seeding density: ≈2 x 105 cells/ml
Recommended Cryopreservation Method
Freeze medium: 70% complete medium + 20% FBS * + 10% DMSO
* One can increase the percentage of FBS upto 50%
Storage temperature: Liquid nitrogen vapor phase
Cell density:
AVAILABILITY
- DSMZ: Product link
- ATCC: Product link
REFERENCES
- Ralph et al., 1974. Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD. Biochem Biophys Res Commun. 61(4), 1268-1275. PMID-4616699; Full-Text Link: sciencedirect
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