Cell Line: P-815

OVERVIEW 
OrganismMurine (mouse) (Mus musculus), ​​DBA/2
Source/Tissue of origin
Mouse mastocytoma tumor
MorphologySingle, round cells
Cell typeMast cell
Growth modeSuspension with some adherent cells
Doubling time
18-22 hours
Life spanInfinite, continuous culture
Karyotype 
Transformed 
Tumorigenic 
Drug resistance 
Biosafety Level1 (dsmz) (May differ in your country, follow the guidelines of your institute/country)
Gene ExpressionLysozyme
Products 
Applications 3D cell culture, transfection host, used as target cells for cytotoxic T cell assays
Remarks 
Related cell lines 

Cell line isolation information

The cell line p-815 was established from the mastocytoma tumor of a DBA/2 mouse treated with methylcolanthrene.

Recommended propagation method

Culture medium:
90% RPMI 1640 + 10% h.i. FBS (dsmz)
90% DMEM + 10% FBS (atcc)

Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Medium Renewal: Every 2 to 3 days

Split ratio: 1:4 to 1:8
Split frequency: every 3-4 days
Harvesting time: > 1 x 106 cells/ml
Split Method: Dilution
Seeding density: ≈2  x 105 cells/ml

Recommended Cryopreservation Method

Freeze medium: 70% complete medium + 20% FBS *  + 10% DMSO
* One can increase the percentage of FBS upto 50%

Storage temperature: Liquid nitrogen vapor phase

Cell density:

AVAILABILITY

REFERENCES

  • Ralph et al., 1974. Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD. Biochem Biophys Res Commun. 61(4), 1268-1275. PMID-4616699; Full-Text Link: sciencedirect

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