Category: Recipes

OVERVIEW 5M hydrochloric acid can be prepared by diluting a concentrated 37% (w/w) stock solution of hydrochloric acid. A 37%…

Hydrochloric acid is supplied as a concentrated stock solution [37% (w/w)]. The molarity of the 37% (w/w)  solution is 12.178…

OVERVIEW Triton X-100 is a non-ionic, mild, non-denaturing detergent, used for solubilizing cell membrane, without denaturing proteins. It is often…

A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.

Magnesium Chloride (MgCl2) is soluble in water. Magnesium chloride is available in anhydrous as well as hydrated forms. Since anhydrous Magnesium chloride is hygroscopic (quickly absorbs water from the air), it is convenient to use hydrated forms that are easy to weigh out the right amount accurately. For this reason, Magnesium chloride hexahydrate (MgCl2.​6H2O, Molecular Weight: 203.30) is often used to prepare a 1 M Magnesium chloride solution. A 1M stock solution can be prepared by dissolving 203.3 g of MgCl2.​6H2O (Molecular Weight: 203.30) in water to a final volume of 100 ml. In the following section, we describe a procedure to prepare 100 ml of 1M MgCl2 stock solution.

A 100 ml of 4% (w/v) paraformaldehyde can be prepared by dissolving 4g of paraformaldehyde in PBS to a final volume of 100 ml.

A 50x TAE buffer can be prepared by mixing and dissolving 242 g Tris base, 100 ml of 0.5 M EDTA and 57.1 ml glacial acetic acid in a deionized water to a final volume of 1000 ml. The pH of the final solution should be between 8.2 – 8.4.