Cell Line : A7r5

OrganismRat (Rattus norvegicus), Strain DB1X
Source/TissueEmbryo, Thoracic aorta, Medial and intimal layers
Cell typeFibroblast, Exhibits some of the properties of muscle cells
Growth modeAdherent
Doubling timeSlow growing
Life span?
Drug resistance?
Biosafety Level1 REF
Gene ExpressionMyokinase, Creatine phosphokinase, Myosin
ApplicationsCalcium channel electrophysiological studies
RemarksSingle cell clone (Clonal cell line)
Related cell lines 

Cell line isolation information

The cell line A7r5 was established from the thoracic aorta of DBIX embryonic rat in 1976.

Recommended propagation method

Culture medium: DMEM (+2mM Glutamine) + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:3 (seeding at 1-3 x 103 cells/cm²)
Split frequency: Once or twice a week
Harvesting time: Subconfluent culture (80%)
Split Method: Trypsin/EDTA

Recommended Cryopreservation Method

Freeze medium: 70% complete culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Cell density: 

Availability (Where to Get the Cell Line)

> American Type Culture Collection (ATCC) : Catalog No.: CRL-1444TM
> Public Health England Culture Collections, Catalogue No.: 86050803
> Sigma-Aldrich, Catalog No.: 86050803
> Cell Bank Australia: Code. : 86050803


> American Type Culture Collection (ATCC)
> Public Health England Culture Collections
> Kimes & Brandt 1976. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp Cell Res. 98(2), 349-366. PMID-943301 | Full Text – Sciencedirect

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