Overview | |
Organism | Rat (Rattus norvegicus), Strain DB1X |
Source/Tissue | Embryo, Thoracic aorta, Medial and intimal layers |
Morphology | Fibroblast-like |
Cell type | Fibroblast, Exhibits some of the properties of muscle cells |
Growth mode | Adherent |
Doubling time | Slow growing |
Life span | ? |
Karyotype | ? |
Transformed | ? |
Tumorigenic | ? |
Drug resistance | ? |
Biosafety Level | 1 REF |
Gene Expression | Myokinase, Creatine phosphokinase, Myosin |
Products | ? |
Applications | Calcium channel electrophysiological studies |
Remarks | Single cell clone (Clonal cell line) |
Related cell lines |
Cell line isolation information
The cell line A7r5 was established from the thoracic aorta of DBIX embryonic rat in 1976.
Recommended propagation method
Culture medium: DMEM (+2mM Glutamine) + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:3 (seeding at 1-3 x 103 cells/cm²)
Split frequency: Once or twice a week
Harvesting time: Subconfluent culture (80%)
Split Method: Trypsin/EDTA
Recommended Cryopreservation Method
Freeze medium: 70% complete culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Cell density:
Availability (Where to Get the Cell Line)
> American Type Culture Collection (ATCC) : Catalog No.: CRL-1444TM
> Public Health England Culture Collections, Catalogue No.: 86050803
> Sigma-Aldrich, Catalog No.: 86050803
> Cell Bank Australia: Code. : 86050803
References
> American Type Culture Collection (ATCC)
> Public Health England Culture Collections
> Kimes & Brandt 1976. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp Cell Res. 98(2), 349-366. PMID-943301 | Full Text – Sciencedirect