| OVERVIEW | |
| Organism | Rat (Rattus norvegicus) |
| Source/Tissue | N-nitrosomethylurea-induced glioma in brain |
| Morphology | Fibroblast-like |
| Cell type | Glial cell |
| Growth mode | Adherent, monolayer |
| Doubling time | 35 – 41 h (Benda et al., 1968) |
| Life span | Infinite, continuous culture |
| Karyotype | Rat hyperdiploid karyotype with 4% polyploidy (dsmz) |
| Transformed | Yes, N-nitrosomethylurea |
| Tumorigenic | Yes |
| Drug resistance | ? |
| Biosafety Level | 1 (ATCC, dsmz) (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | |
| Products | ? |
| Applications | 3D cell culture |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line C6 was established from rat glial tumor induced by N-nitrosomethylurea. (Benda et al., 1968)
Recommended propagation method
Culture medium: 82.5% Ham’s F12 + 15% horse serum + 2.5% h.i. FBS + 2 mM L-glutamine (dsmz)
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio:
Split frequency:
Harvesting time:
Split Method: Trypsin/EDTA
Recommended Cryopreservation Method
Freeze medium: 70% complete culture medium + 20% FBS * + 10% DMSO (dsmz)
* One can increase the percentage of FBS upto 50%.
Storage temperature: Liquid nitrogen vapor phase REF
Cell density:
AVAILABILITY
♦ American Type Culture Collection (ATCC) : Product link
♦ DSMZ : Catalog No. : Product link
♦ Sigma-Aldrich: Product link
♦ JCRB cell Bank: Product link, Product link
REFERENCES
- Benda et al., 1968. Differentiated rat glial cell strain in tissue culture. Science. 161(3839), 370-271. PMID-4873531; Full Text Link: Science, booksc
