Cell Line : D283 Med

Overview

 
OrganismHuman (Homo sapiens), 6 years old, Male, White (Friedman et al., 1985)
Source/TissueBrain, Cerebellum, Medulloblastoma REF
MorphologyEpithelial REF
Cell type 
Growth modemulticellular aggregates, some adherent REF
Doubling time 52.55 h (Friedman et al., 1985)
Life spanInfinite, continuous culture (Friedman et al., 1985)
KaryotypeHypodiploid REF
TransformedNaturally transformed (anchorage independent growth)
TumorigenicTumorigenic in nude mice (Friedman et al., 1985)
Drug resistance 
Biosafety Level1 REF (May differ in your country, follow the guidelines of your institute/country)
Gene Expression 
Products 
Applications

3D cell culture REF
Neuroscience REF

Remarks 
Related cell lines 

Cell line isolation information

The cell line D283 Med was established from malignant ascites cells and a peritoneal metastasis from a 6 years old boy with medulloblastoma. (Friedman et al., 1985)

Recommended propagation method

Culture medium:
Eagle’s Minimum Essential Medium (+ 2mM Glutamine) + 10% FBS REF

Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C REF
Split ratio:
Split frequency:
Harvesting time: Subconfluent culture (≈ 80% )
Split Method: Dilution REF

Recommended Cryopreservation Method

Freeze medium: 70% complete culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase / -150 °C freezer
Cell density: ?

Availability

REFERENCES

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