|Organism||Human (Homo sapiens), 42 years old Male|
|Source/Tissue of origin||Thyroid, Lymph node metastasis, Follicular thyroid carcinoma|
|Morphology||Spindle- or round-shaped cells|
|Growth mode||Adherent, monolayer|
|Life span||Infinite, continuous culture|
|Biosafety Level||2 (phe-culturecollections) (May differ in your country, follow the guidelines of your institute/country)|
|Gene Expression||P53 mutation, Y-chromosome is lost|
|Related cell lines|
Cell line isolation information
The cell line FTC-133 was established from a lymph node metastasis of a follicular thyroid carcinoma from a 42-year-old male.
Recommended propagation method
Culture medium: DMEM : Ham’s F12 (1:1) + 2mM Glutamine + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:8 to 1:12
Harvesting time: Subconfluent culture (≈80%)
Split Method: Trypsin/EDTA
Seeding density: 10,000 – 50,000 cells/ cm2
Recommended Cryopreservation Method
Freeze medium: 40% complete culture medium + 50% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase
- Public Health England : Product link
- Cell Bank Australia: Product link
- Hölting et al., 1994. Transforming growth factor-beta 1 is a negative regulator for differentiated thyroid cancer: studies of growth, migration, invasion, and adhesion of cultured follicular and papillary thyroid cancer cell lines. J Clin Endocrinol Metab. 79(3), 806-813. PMID-8077365; Full Text Link: academic.oup
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