Cell Line: FTC-133

OrganismHuman (Homo sapiens), 42 years old Male
Source/Tissue of origin
Thyroid, Lymph node metastasis, Follicular thyroid carcinoma
MorphologySpindle- or round-shaped cells
Cell type 
Growth modeAdherent, monolayer
Doubling time
Life spanInfinite, continuous culture
Drug resistance 
Biosafety Level2 (phe-culturecollections) (May differ in your country, follow the guidelines of your institute/country)
Gene ExpressionP53 mutation, Y-chromosome is lost
Related cell lines 

Cell line isolation information

The cell line FTC-133 was established from a lymph node metastasis of a follicular thyroid carcinoma from a 42-year-old male.

Recommended propagation method

Culture medium: DMEM : Ham’s F12 (1:1) + 2mM Glutamine + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:8 to 1:12
Split frequency:
Harvesting time: Subconfluent culture (≈80%)
Split Method: Trypsin/EDTA
Seeding density: 10,000 – 50,000 cells/ cm2

Recommended Cryopreservation Method

Freeze medium: 40% complete culture medium + 50% FBS  + 10% DMSO

Storage temperature: Liquid nitrogen vapor phase

Cell density:



  • Hölting et al., 1994. Transforming growth factor-beta 1 is a negative regulator for differentiated thyroid cancer: studies of growth, migration, invasion, and adhesion of cultured follicular and papillary thyroid cancer cell lines. J Clin Endocrinol Metab. 79(3), 806-813. PMID-8077365; Full Text Link: academic.oup

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