- Synonyms:
- Culture Type: Cell line
- Keywords:
- Description: EBV transformed peripheral blood lymphocytes. The donor was suffering from TNM stage IIA, grade 3, primary invasive ductal carcinoma.
- Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: 41 years
- Ethnicity: White
- Gender: Female
- Disease: Normal
- Tissue of origin: Peripheral blood
- Year of origin: –
- Organism:
- Cell line features:
- Morphology: Lymphoblast
- Cell Type: B lymphoblast
- Growth mode: Suspension, multicellular aggregates
- Life span: Infinite (continuous culture)
- EBV transformed
- Karyotype: –
- Doubling time: –
- Gene mutations:
- –
- Gene expression:
- –
- Morphology: Lymphoblast
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
- Split ratio:-
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freeze medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 2 – 4 x 106 viable cells/ml
- Volume per vial: 1 ml
- Freeze medium: Complete growth medium + 5% DMSO
- Applications:
- Immunology
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- https://www.atcc.org/products/crl-2323
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774. PMID-9833771; Full-Text Links: Wiley
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- EBV (Epstein-Barr virus)
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