- Synonyms:
- Culture Type: Cell line
- Keywords:
- Description: Cells were established from a primary ductal carcinoma derived from a 23-year-old, White female with stage IIB breast cancer.
- Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: 23 years
- Ethnicity: White
- Gender: Female
- Disease: Ductal Carcinoma TNM Stage IIB, grade 3
- Tissue of origin: Mammary gland Duct, Breast
- Year of origin:
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial cell
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Modal chromosome number: 100
- Doubling time:
- Gene mutations:
- Homozygous for the BRCA1 5382C mutation
- TP53 mutation
- Homozygous deletion of the PTEN gene
- Clinically relevant gene expression
- Epithelial glycoprotein 2 positive
- Cytokeratin 19 positive
- Estrogen receptor negative
- Progesterone receptor negative
- Her2-neu negative
- p53 negative
- Epithelial glycoprotein 2 positive
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: Sub-confluent cultures (≈80% confluent)
- Split ratio: 1:2 to 1:4
- Seeding densities:
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freeze medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cells suspension (cell density): 2 – 4 x 106 viable cells/ml
- Volume per vial: 1 ml
- Freeze medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- https://www.atcc.org/products/crl-2336
- Tomlinson et al., 1998. Characterization of a breast cancer cell line derived from a germ-line BRCA1 mutation. Cancer Res. 58: 3237-3242. PMID-9699648; Full-Text Links: Sciencedirect, Researchgate (Download PDF)
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PMID-9833771; Full-Text Links: Wiley (Download PDF)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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