OVERVIEW | |
Organism | Human (Homo sapiens), 15 years old white male (Ref: ATCC) |
Source/Tissue of origin | Liver (Ref: ATCC) Established from hepatocellular carcinoma (Ref: ATCC) |
Year of isolation | 1975 (Ref: ATCC) |
Morphology | Epithelial-like (Ref: ATCC) |
Cell type | Epithelial (Ref: ATCC) |
Growth mode | Adherent (Ref: ATCC) |
Doubling time | ? |
Life span | Infinite, continuous culture (Ref: ATCC) |
Karyotype | Modal chromosome number = 55 (Ref: ATCC) |
Transformed | Naturally transformed (can form colonies in semisolid medium) (Ref: ATCC) |
Tumorigenic | Yes (in nude mice) (Ref: ATCC) |
Drug resistance | ? |
Gene Expression | |
Positive for | Albumin (Ref: ATCC) |
Altered gene | |
Enzymes | |
Products | |
Applications | 3D cell culture (Ref: ATCC) Suitable transfection host (Ref: ATCC) Cancer research (Ref: ATCC) High-throughput screening (Ref: ATCC) |
Remarks | |
Keywords | Human liver cancer cell line |
Related cell lines | |
Culture Maintenance | |
Biosafety Level | BSL 1 (Ref: ATCC) (It may differ in your country, follow the guidelines of your institute/country) |
Culture medium | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FBS (Ref: ATCC) |
Culture conditions | Humidified incubator, 37°C, 5% CO2 (Ref: ATCC) |
Subculturing procedure | Trypsin-EDTA |
Culture storage | |
Cryo-medium | Complete growth medium + 5% (v/v) (Ref: ATCC) |
Storage conditions | Vapor phase of liquid nitrogen |
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