Cell Line: Hep G2

OrganismHuman (Homo sapiens), 15 years old white male (Ref: ATCC)
Source/Tissue of originLiver (Ref: ATCC)
Established from hepatocellular carcinoma (Ref: ATCC)
Year of isolation1975 (Ref: ATCC)
MorphologyEpithelial-like (Ref: ATCC)
Cell typeEpithelial (Ref: ATCC)
Growth modeAdherent (Ref: ATCC)
Doubling time?
Life spanInfinite, continuous culture (Ref: ATCC)
KaryotypeModal chromosome number = 55 (Ref: ATCC)
TransformedNaturally transformed (can form colonies in semisolid medium) (Ref: ATCC)
TumorigenicYes (in nude mice) (Ref: ATCC)
Drug resistance?
Gene Expression
Positive for

Albumin (Ref: ATCC)
Alpha-fetoprotein (AFP) (Ref: ATCC)
Insulin (Ref: ATCC)
Insulin-like growth factor II (IGF II) (Ref: ATCC)

Altered gene 
Applications3D cell culture (Ref: ATCC)
Suitable transfection host (Ref: ATCC)
Cancer research (Ref: ATCC)
High-throughput screening (Ref: ATCC)
KeywordsHuman liver cancer cell line
Related cell lines 
Culture Maintenance
Biosafety LevelBSL 1 (Ref: ATCC)
(It may differ in your country, follow the guidelines of your institute/country)
Culture mediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FBS (Ref: ATCC)
Culture conditionsHumidified incubator, 37°C, 5% CO2 (Ref: ATCC)
Subculturing procedureTrypsin-EDTA
Culture storage
Cryo-mediumComplete growth medium + 5% (v/v) (Ref: ATCC)
Storage conditionsVapor phase of liquid nitrogen


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