- Synonyms:
- Culture Type: Cell line
- Keywords: BMI1-immortalized primary human pulmonary endothelial cells
- Description: Cells possess key endothelial cell characteristics
- Expression of CD31/ PECAM-1
- Uptake of acetylated low-density lipoprotein
- form capillary-like tubes on a basement membrane matrix
- Source:
- Organism:
- Species: Human (Homo sapiens)
- Ethnicity: White
- Gender: Male
- Tissue: Pulmonary artery
- Year of origin: 2022
- Organism:
- Cell line features:
- Morphology: Endothelial-like
- Cell Type: Endothelial
- Growth mode: Adherent, Monolayer
- Life span: Infinite (continuous culture)?
- Karyotype: Near Diploid
- Doubling time: ≈30 hrs
- Morphology: Endothelial-like
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Vascular Cell Basal Medium (ATCC PCS-100-030) + Endothelial Cell Growth Kit-BBE (ATCC PCS-100-040) + 4 µg/mL Blasticidin
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin-EDTA
- Harvesting time: Sub-confluent cultures (when cell density reaches 3.8 x 104 cell/cm2)
- Split ratio: 1:5 to 1:9
- Seeding densities: 4 x 103 and 5 x 103 viable cells/cm2
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freeze medium: Stem Cell Freezing Media (ATCC ACS-3020)
- Storage conditions: Vapor phase of liquid nitrogen
- Cells suspension (cell density): 1.0 – 1.5 x 106 viable cells/ml
- Volume per vial: 1 ml
- Freeze medium: Stem Cell Freezing Media (ATCC ACS-3020)
- Applications:
- Cancer research
- Toxicology research
- Cardiovascular disease research
- Drug development
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Abbreviations
- BSL (Biosafety level)
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