| OVERVIEW | |
| Organism | Mouse (Mus musculus), 100-day-old male, C3H/An |
| Source/Tissue of origin | Subcutaneous connective tissue, Adipose, Areolar |
| Morphology | Fibroblasts-like |
| Cell type | Connective tissue fibroblasts |
| Growth mode | Adherent, monolayer |
| Doubling time | 20-24 hours |
| Life span | Infinite, continuous culture |
| Karyotype | Modal chromosome number = 66; range = 65 to 68 |
| Transformed | |
| Tumorigenic | Tumorigenic in immunosuppressed mice |
| Drug resistance | |
| Biosafety Level | 1 (dsmz/atcc) (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | |
| Products | |
| Applications | 3D cell culture, toxicity testing |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line L-929 was established from the normal subcutaneous areolar and adipose tissue of a male C3H/An mouse.
Recommended propagation method
Culture medium:
1. 90% RPMI 1640 + 10% h.i. FBS (dsmz)
2. 90% Eagle’s Minimum Essential Medium + 10% horse serum (atcc)
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:5 to 1:10
Split frequency: 2-3 times a week
Harvesting time: Confluent culture
Split Method: Trypsin (do not use trypsin/EDTA) (dsmz)
Seeding density: 2.0-3.0 x 106 cells/80 cm2
Recommended Cryopreservation Method
Freeze medium:
1. 70% complete medium + 20% FBS + 10% DMSO (dsmz)
2. Complete growth medium supplemented with 5% (v/v) DMSO (atcc)
Storage temperature: Liquid nitrogen vapor phase
Cell density:
AVAILABILITY
- Public Health England : Product link
- Cell Bank Australia: Product link
REFERENCES
- Sanford et al., 1948. The growth in vitro of single isolated tissue cells. J Natl Cancer Inst. 9(3), 229-246. PMID-18105872
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