- Synonyms:
- Culture Type: Cell line
- Keywords: Luciferase reporter-labeled cell line
- Description:
- Parental line: MG-63
- Stably expressing firefly luciferase gene (luc2) under the control of a GAS promoter
- Parental line: MG-63
- Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: 14 years
- Ethnicity: White
- Gender: Male
- Disease: Osteosarcoma
- Tissue of origin: Bone
- Year of origin: 2022
- Organism:
- Cell line features:
- Morphology: Fibroblast-like
- Cell Type: ?
- Growth mode: Adherent, Monolayer
- Life span: Infinite (continuous culture)
- Karyotype: hypotriploid, Modal chromosome number: 66
- Doubling time: ?
- Morphology: Fibroblast-like
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: EMEM + 10% heat-inactivated FBS + 0.5 µg/mL puromycin
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: Sub-confluent cultures (when cell density reaches 5 x 104 cell/cm2)
- Split ratio: 1:4 to 1:8
- Seeding densities: 5 x 103 and 7 x 103 viable cells/cm2
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freeze medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cells suspension (cell density): 6 – 8 x 106 viable cells/ml
- Volume per vial: 1 ml
- Freeze medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- Immuno-oncology
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Abbreviations
- BSL (Biosafety level)
- EMEM (Eagle’s Minimum Essential Medium)
- FBS (Fetal Bovine Serum)
- GAS (Gamma-activated site)
- DMSO (Dimethyl sulfoxide)
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