- Cell Line: SW1271
- Synonyms:
- SW 1271
- SW-1271
- Culture Type: Cell line
- Keywords: Human epithelial small cell lung carcinoma cell line
- Description: Established from the lung of a 69-year-old, White male patient with small cell lung cancer
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 69 years
- Ethnicity: White
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Lung
- Disease:
- Small Cell Lung Cancer
- Primary/Metastasis: Primary
- Year of origin: 1976
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Gene expression:
- Positive for Blood Type A and Rh (Antigen)
- Sequence variations (gene mutations/deletions/fusions/translocations):
- NRAS (Q61R mutation, Oncogenic Gain-of-function)
- TP53 (C277F mutation, Likely Oncogenic, Likely Loss-of-function)
- For more information, please visit
- https://depmap.org/portal/cell_line/SW1271_LUNG?tab=mutations
- https://depmap.org/portal/cell_line/SW1271_LUNG?tab=fusions
- https://depmap.org/portal/cell_line/SW1271_LUNG?tab=translocations
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Leibovitz’s L-15 Medium + 10% FBS (ATCC)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 100% Air (no CO2)
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:
- Split ratio: 1:4 to 1:6
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO??????
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- Addexbio Technologies: Product Code: Product link
- Caltag Medsystems Limited: Product Code: Product link
- References
- Images
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
