- Cell Line: SW1271
- Synonyms:
- SW 1271
- SW-1271
- Culture Type: Cell line
- Keywords: Human epithelial small cell lung carcinoma cell line
- Description: Established from the lung of a 69-year-old, White male patient with small cell lung cancer
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 69 years
- Ethnicity: White
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Lung
- Disease:
- Small Cell Lung Cancer
- Primary/Metastasis: Primary
- Year of origin: 1976
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Gene expression:
- Positive for Blood Type A and Rh (Antigen)
- Sequence variations (gene mutations/deletions/fusions/translocations):
- NRAS (Q61R mutation, Oncogenic Gain-of-function)
- TP53 (C277F mutation, Likely Oncogenic, Likely Loss-of-function)
- For more information, please visit
- https://depmap.org/portal/cell_line/SW1271_LUNG?tab=mutations
- https://depmap.org/portal/cell_line/SW1271_LUNG?tab=fusions
- https://depmap.org/portal/cell_line/SW1271_LUNG?tab=translocations
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Leibovitz’s L-15 Medium + 10% FBS (ATCC)
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air (no CO2)
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:
- Split ratio: 1:4 to 1:6
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO??????
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- Addexbio Technologies: Product Code: Product link
- Caltag Medsystems Limited: Product Code: Product link
- References
- Images
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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