CspCI Restriction Enzyme

  • Endonuclease
  • Type IIS Restriction enzyme
  • Cleaves double-stranded DNA outside of specific recognition site
  • Recognizes asymmetric DNA sequences (see fig)
  • Cleaves on both sides of recognition sequences, resulting in elimination of recognition site
  • Requires both S-Adenosyl methionine and Mg2+ as a cofactor
  • DNA Sticky end cutter (cleaves both strands of the DNA at different locations outside recognition site, resulting in sticky overhang end)
  • Generates 3’overhang

CspC1 Restriction Enzyme


From New England BioLabs
Specifications: CspCI
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