Dulbecco’s Modified Eagle’s Medium (DMEM)

Dulbecco’s modified Eagle’s medium is extensively used for animal tissue culture.

Composition of Dulbecco’s modified Eagle’s medium
Inorganic Salts
Calcium Chloride (CaCl2) (anhydrous)200 mg/L
Ferric Nitrate (Fe(NO3)3.9H2O)0.1 mg/L
Magnesium Sulfate (MgSO4.7H2O)200 mg/L
Potassium Chloride (KCl)400 mg/L
Sodium Bicarbonate (NaHCO3)3700 mg/L
Sodium Chloride (NaCl)6400 mg/L
Sodium Phosphate monobasic (NaH2PO4.H2O)125 mg/L
Sodium pyruvate110 mg/L
Amino Acids
L-Arginine.HCl84 mg/L
L-Cystine *48 mg/L
L-Histidine.HCl.H2O42 mg/L
L-Isoleucine105 mg/L
L-Glutamine580 mg/L
Glycine30 mg/L
L-Leucine105 mg/L
L-Lysine.H2O *150 mg/L
L-Methionine30 mg/L
L-Phenylalanine66 mg/L
L-Serine42 mg/L
L-Threonine95 mg/L
L-Tryptophan16 mg/L
L-Tyrosine72 mg/L
L-Valine94 mg/L
D-Calcium pantothenate4 mg/L
Choline chloride4 mg/L
Folic acid4 mg/L
i-Inositol7.2 mg/L
Nicotinamide4 mg/L
Pyridoxal.HCl4 mg/L
Riboflavin0.4 mg/L
Thiamine HCl4 mg/L
Other components
Glucose4500 mg/L
Phenol Red15 mg/L

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