| OVERVIEW | |
| Organism | Human (Homo sapiens), 36 years, White, Female |
| Source/Tissue of origin | Peripheral blood, Acute promyelocytic leukemia |
| Morphology | Lymphoblast-like |
| Cell type | |
| Growth mode | Suspension |
| Doubling time | |
| Life span | Infinite, continuous culture |
| Karyotype | |
| Transformed | |
| Tumorigenic | Tumorigenic in nude mice |
| Drug resistance | |
| Biosafety Level | 2 (atcc) (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | |
| Products | |
| Applications | |
| Remarks | HL-60/MX2 cells can tolerate upto 190 nM mitoxantrone. |
| Related cell lines |
Cell line isolation information
The cell line HL-60/MX2 is a mitoxantrone resistant (39 nM mitoxantrone) derivative of the HL-60 cell line. HL-60 cells were established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976. The HL-60/MX2 cells exhibit a reciprocal translocation [rcpt(1;3)-(q21;p23)] not found in the parental HL-60 cell line.
Recommended propagation method
Culture medium: RPMI 1640 + 10% FBSS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:4
Split frequency:
Harvesting time: when the cell density is 1.5 x 106 cells/ml
Split Method: Dilution
Seeding density: 1 – 2 x 105 cells/ml
Recommended Cryopreservation Method
Freeze medium:
Complete culture medium + 20% FBS * + 10% DMSO (* One can increase the percentage of FBS upto 50%)
Complete growth medium supplemented with 5% (v/v) DMSO (atcc)
Storage temperature: Liquid nitrogen vapor phase
Cell density:
AVAILABILITY
- ATCC : Product link
REFERENCES
- Harker et al., 1989. Multidrug resistance in mitoxantrone-selected HL-60 leukemia cells in the absence of P-glycoprotein overexpression. Cancer Res. 49(16), 4542-4549. PMID-2568172; Full-Text Link: aacrjournals (download PDF)
