OrganismHuman (Homo sapiens), 36 years, White, Female
Source/Tissue of origin
Peripheral blood, Acute promyelocytic leukemia
Cell type 
Growth modeSuspension
Doubling time
Life spanInfinite, continuous culture
Tumorigenic Tumorigenic in nude mice
Drug resistance 
Biosafety Level2 (atcc) (May differ in your country, follow the guidelines of your institute/country)
Gene Expression 
RemarksHL-60/MX2 cells can tolerate upto 190 nM mitoxantrone.
Related cell lines 

Cell line isolation information

The cell line HL-60/MX2 is a mitoxantrone resistant (39 nM mitoxantrone) derivative of the HL-60 cell line. HL-60 cells were established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976. The HL-60/MX2 cells exhibit a reciprocal translocation [rcpt(1;3)-(q21;p23)] not found in the parental HL-60 cell line.

Recommended propagation method

Culture medium: RPMI 1640 + 10% FBSS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:4
Split frequency:
Harvesting time: when the cell density is 1.5 x 106 cells/ml
Split Method: Dilution
Seeding density: 1 – 2 x 105 cells/ml

Recommended Cryopreservation Method

Freeze medium:
Complete culture medium + 20% FBS *  + 10% DMSO (* One can increase the percentage of FBS upto 50%)
Complete growth medium supplemented with 5% (v/v) DMSO (atcc)

Storage temperature: Liquid nitrogen vapor phase

Cell density:



  • Harker et al., 1989. Multidrug resistance in mitoxantrone-selected HL-60 leukemia cells in the absence of P-glycoprotein overexpression. Cancer Res. 49(16), 4542-4549. PMID-2568172; Full-Text Link: aacrjournals (download PDF)

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