|Organism||Human (Homo sapiens), 36 years old white female|
|Year of isolation|
|Source/Tissue of origin||Peripheral blood, Acute promyelocytic leukemia|
|Doubling time||about 40 hours (dsmz)|
|Life span||Infinite, continuous culture|
|Tumorigenic|| Tumorigenic in nude mice|
|Biosafety Level||1 (dsmz, atcc) (May differ in your country, follow the guidelines of your institute/country)|
|Remarks||Differentiate spontaneously. |
Differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid, dimethylsulfoxide, actinomycin D, and retinoic acid.
|Related cell lines|
Cell line isolation information
The cell line HL-60 was established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976.
Recommended propagation method
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:5
Split frequency: every 2-3 days
Harvesting time: 1 X 106 (Do not allow cell concentration to exceed 1 X 106 (atcc))
Split Method: Dilution
Seeding density: 1 X 105/ml
Recommended Cryopreservation Method
Storage temperature: Liquid nitrogen vapor phase
Cell density: 1 X 106 cells /ml
- Gallagher et al., 1979. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood. 54(3), 713-733. PMID-288488; Full-text Link: sciencedirect (download PDF)