Cell Line: HL-60

OrganismHuman (Homo sapiens), 36 years old white female
Year of isolation
Source/Tissue of origin
Peripheral blood, Acute promyelocytic leukemia
Cell typePromyeloblast
Growth modeSuspension
Doubling time
about 40 hours (dsmz)
Life spanInfinite, continuous culture
Karyotype Pseudodiploid
Tumorigenic Tumorigenic in nude mice
Drug resistance 
Biosafety Level1 (dsmz, atcc) (May differ in your country, follow the guidelines of your institute/country)
Gene ExpressionTNF-alpha
RemarksDifferentiate spontaneously.
Differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid, dimethylsulfoxide, actinomycin D, and retinoic acid.
Related cell lines 

Cell line isolation information

The cell line HL-60 was established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976.

Recommended propagation method

Culture medium:
1. RPMI 1640 + 10% h.i. FBS (dsmz)
2. Iscove’s Modified Dulbecco’s Medium + 20% FBS (atcc)

Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:5
Split frequency: every 2-3 days
Harvesting time: 1 X 106 (Do not allow cell concentration to exceed 1 X 106 (atcc))
Split Method: Dilution
Seeding density: 1 X 105/ml

Recommended Cryopreservation Method

Freeze medium:
1. complete medium + 20% FBS  + 10% DMSO (dsmz)
2. Iscove’s Modified Dulbecco’s Medium + 7% FBS + 5% (v/v) DMSO (atcc)

Storage temperature: Liquid nitrogen vapor phase

Cell density: 1 X 106 cells /ml



  • Gallagher et al., 1979. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood. 54(3), 713-733. PMID-288488; Full-text Link: sciencedirect (download PDF)

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