How to Study Changes in Buffer pH During Agarose Gel Electrophoresis?

Changes in electrophoresis buffer pH is an important parameter to consider when optimizing already existing reagents or conditions or testing new reagents/conditions for agarose gel electrophoresis. 

To analyze the buffer pH change, take a few milliliter (≈ 2 ml) of electrophoresis buffer from both anode and cathode chamber and analyze their pH by pH meter.

REFERENCES:

Sanderson et al., 2014. Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis. Anal Biochem. 454, 44-52. PMID-24637158; Full-Text Links: sciencedirect, PMC4021863

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