KOD DNA Polymerase

  • Also known as KOD HiFi DNA Polymerase
  • High-fidelity thermostable DNA polymerase for error-free polymerase chain reaction.
  • Originally isolated from Thermococcus kodakaraensis (originally named Pyrococcus kodakarensis KOD1) (Takagi et al., 1997), recombinant enzyme is expressed and isolated from E. coli.
  • Suitable for long template PCR reaction (amplification of upto 6 kb long fragments with high fidelity)
  • Better elongation rate (5 times higher) and processivity (10-15 times higher) than pfu enzyme, makes KOD DNA polymerase a better choice for accurate error-free amplification of long DNA templates
  • Possess 3′ to 5′ exonuclease activity for proofreading activity
  • Produces blunt-end DNA fragments


  • Takagi et al., 1997. Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR.  Appl Environ Microbiol. 63(11), 4504-4510. asm (download PDF), PMC168769 (download PDF)

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