# Overview:

A 1M Acetic acid solution can be prepared by diluting the concentrated acetic acid stock solution. Glacial acetic acid, a highly concentrated solution of acetic acid, is available from several commercial suppliers and contains ≈99.7% (w/w) acetic acid. The molarity of this solution is 17.416 M (see the calculation procedure). To prepare the 1000 ml of 1M acetic acid solution, 57.42 ml of glacial acetic acid is mixed with  942.58 ml of water.

# REQUIREMENTS

Reagents and solutions
Glacial acetic acid (99.7%)
Deionized / Milli-Q water

Equipment and disposables

Composition
1 M Acetic acid solution in water

Objective
Preparation of 1 M stock solution of Acetic acid using glacial acetic acid (Conc: 99.7% or 17.416M)

# PREPARATION

Step 1: Calculate the amount of glacial acetic acid required for the preparation of 1000 ml of 1 M acetic acid solution.

Formula : C1 x V1 = C2 x V2

Known values:

C1 = 17.416 (Molarity of glacial acetic acid)
V1 = not known (Required volume of glacial acetic acid)
C2 = 1 M (concentration of final acetic acid solution)
V2 = 1000 ml (volume of final acetic acid solution)

Place all values in the above formula:

17.416 x V1 = 1 x 1000
V1 = 1 x 1000/17.416 = 57.42

So the amount of glacial acetic acid required for the preparation of 1 M Acetic acid solution = 57.42

Volume of water required = 1000 (final volume of the solution) – 57.42 (volume of glacial acetic acid) = 942.58 ml

So we need to mix 57.42 ml glacial acetic acid in 942.58 ml of water to prepare 100 ml of 1M acetic acid solution.

Step 2: Transfer 57.42 ml glacial acetic acid in a volumetric flask/measuring cylinder and make up the final volume to 1000 ml by adding 942.58 ml water.

Step 3: Close the lid tightly and mix by inversion.

Storage:
1M Acetic acid solution can be stored at room temperature for several months.