Potassium Chloride (KCl) dissolves readily in water. A 1 M stock solution can be prepared by dissolving 74.55 g KCL (Molecular Weight: 74.55) in water to a final volume of 1L.
In the following section, we describe a procedure to prepare 100 ml of 1M KCl stock solution.
Reagents and solutions
Potassium chloride (KCl)
Deionized / Distilled water
Equipment and disposables
Conical flask / Beaker
1 M Potassium chloride
Preparation of 100 ml 1 M Potassium chloride
Step 1: To prepare 100 ml of 1 M Potassium chloride, weigh out 7.455 g KCL (Molecular Weight: 74.55) in a 250 ml conical flask/beaker. Add 800 ml deionized/Milli-Q water and mix it.
One can use manual shaking using a glass pipette to mix the ingredients. Magnetic stirrer makes the dissolving process easy and convenient.
Do not dissolve in 100 ml of deionized / Milli-Q water. In most cases, solution volume increases when a large amount of solute is dissolved in a solvent.
Step 2: Adjust the volume to 100 ml with deionized / Milli-Q water. Mix it again.
Step 3: Sterilize the solution by autoclaving (20 minutes at 15 lb/sq.in. (psi) from 121-124°C on liquid cycle).
The solution can be stored at room temperature or in a cold room.
|Follow the table to prepare Potassium chloride of various concentrations and volumes (10, 100 ml, 500 ml, 1,000 ml, and 10,000 ml).|
|Solution concentration / Volume||10 ml||100 ml||500 ml||1000 ml|
|0.1 M||0.075 g||0.75 g||3.73 g||7.46 g|
|0.5 M||0.37 g||3.73 g||18.64 g||37.275 g|
|1 M||0.75 g||7.46 g||37.275 g||74.55 g|