Overview:
Potassium Chloride (KCl) dissolves readily in water. A 1 M stock solution can be prepared by dissolving 74.55 g KCL (Molecular Weight: 74.55) in water to a final volume of 1L.
In the following section, we describe a procedure to prepare 100 ml of 1M KCl stock solution.
REQUIREMENTS
Reagents and solutions
Potassium chloride (KCl)
Deionized / Distilled water
Equipment and disposables
Measuring cylinder
Conical flask / Beaker
Composition
1 M Potassium chloride
Objective
Preparation of 100 ml 1 M Potassium chloride
PROCEDURE
Step 1: To prepare 100 ml of 1 M Potassium chloride, weigh out 7.455 g KCL (Molecular Weight: 74.55) in a 250 ml conical flask/beaker. Add 80 ml deionized/Milli-Q water and mix it.
Tip:
One can use manual shaking using a glass pipette to mix the ingredients. Magnetic stirrer makes the dissolving process easy and convenient.
Precaution:
Do not dissolve in 100 ml of deionized / Milli-Q water. In most cases, solution volume increases when a large amount of solute is dissolved in a solvent.
Step 2: Adjust the volume to 100 ml with deionized / Milli-Q water. Mix it again.
Step 3: Sterilize the solution by autoclaving (20 minutes at 15 lb/sq.in. (psi) from 121-124°C on liquid cycle).
Storage
The solution can be stored at room temperature or in a cold room.
Follow the table to prepare Potassium chloride of various concentrations and volumes (10, 100 ml, 500 ml, 1,000 ml, and 10,000 ml). | ||||
Solution concentration / Volume | 10 ml | 100 ml | 500 ml | 1000 ml |
0.1 M | 0.075 g | 0.75 g | 3.73 g | 7.46 g |
0.5 M | 0.37 g | 3.73 g | 18.64 g | 37.275 g |
1 M | 0.75 g | 7.46 g | 37.275 g | 74.55 g |
Preparation of KCL itself in a special container! Solution used! The rest, the precipitate of KCL that has not dissolved! Is it worth using it again?