Preparation of 1M Sulfuric Acid from Concentrated Stock Solution (95%, w/w)


A 95% (w/w) Sulfuric acid can be purchased from several commercial suppliers. It is a clear colorless liquid and can be diluted to prepare solutions of known concentrations. Here we describe a procedure to prepare a 1 M Sulfuric acid solution using commercially supplied 95% stock solution.


Reagents and solutions
95% (w/w) Sulfuric acid solution
Distilled water

Equipment and disposables
Measuring cylinder/volumetric flask
Beaker (1 Liter)
Magnetic stirrer

Preparation of 1 Liter of 1M Sulfuric acid using concentrated stock solution (95%, w/w)


Use personal protective equipment (lab coat, gloves, goggles etc) for your safety and follow the guidelines of your institute.

PART I: Calculate how much 95% (w/w) concentrated stock Solution is required to prepare 1 L of 1M Sulfuric acid solution

Step 1: Calculate the molarity of 95% (w/w) concentrated Sulfuric acid solution
The procedure to calculate molarity of 95% (w/w) concentrated Sulfuric acid solution is described in detail in the post “Molarity of 95% (w/w) Sulfuric acid (H2SO4)”. From this post, you will know that the molarity of 95% (w/w) concentrated Sulfuric acid solution is 17.82 M.

Step 2: Calculate how much concentrated stock solution is required to prepare 1L of 1M Sulfuric acid solution using dilution method.

Dilution method is described in detail in the post “Preparing Working Solution from Stock Solution by Dilution”.

Use the following formula to calculate the volume of concentrated solution required for the preparation of 1 liter of 1M solution of hydrochloric acid solution

Formula:   Cf x Vf = CS x VS

Cf (concentration of diluted (final) solution) = 1M
Vf (volume of diluted (final) solution) = 1 liter
CS (concentration of stock solution) = 17.82 M
VS (volume of stock solution) = ? (unknown)

Place all values in the formula:

1 x 1 = 17.82 x VS

VS = 1 x 1/ 17.82 = 0.056 liter (56 ml)

So you need 0.056 liters of 95% (w/w) concentrated Sulfuric acid solution to prepare a 1 liter of 1M Sulfuric acid solution.

Step 3: Calculate the volume of solvent to be taken.

Final volume of diluted solution = 1Liter
Volume of concentrated solution = 0.056 ml
Volume of solvent (water) required = 1 – 0.056 = 0.944 liter

So you need to mix 0.056 liter (i.e., 56 ml) of concentrated solution to 0.944 liter (944 ml) water to prepare 1 liter of 1M hydrochloric acid solution.

PART II: Solution preparation

Step 4: Transfer 944 ml water in a beaker/volumetric flask/measuring cylinder. Add 56 ml of 95% Sulfuric acid stock solution and mix it to prepare a homogeneous solution.
Note: In case you have not calculated the amount of water to be added, just take ≈ 500 ml water in a volumetric flask/measuring cylinder (capacity 1L) and add 56 ml of  95% Sulfuric acid stock solution. Now adjust the final volume to 1 liter with water. Mix it.

Solution can be stored at room temperature.

Was this post helpful?

Author: admin

Leave a Reply

Your email address will not be published. Required fields are marked *