Preparation of 20% Glucose Stock Solution

Overview:

Glucose is a carbohydrate and is utilized by cells as instant energy and carbon source. In addition, it is also added in solution to maintain the osmolarity of the solution. A 20% glucose stock solution can be prepared by dissolving 20 g of Glucose in distilled/deionized water to a final volume of 100 ml. Working glucose solution is prepared by diluting this stock solution. 

Since glucose is susceptible to degradation at high temperatures (Woo et al., 2015), the glucose solution is sterilized by filtering through a filter unit containing a membrane with pore size 0.22 µm or less under sterile conditions. 

REQUIREMENTS

Reagents and solutions
Glucose (C₆H₁₂O₆)
Deionized or Milli-Q water

Equipment and disposables
Measuring cylinder
Conical flask / Beaker
Magnetic stirrer

Composition
20% Glucose

Objective
Preparation of 100 ml of 20% Glucose stock solution

PROCEDURE

Step 1: To prepare 100 ml of 20% Glucose solution, weigh out 20 g Glucose in a 250 conical flask/beaker. Add 80 ml deionized/Milli-Q water. Stir until all glucose is completely dissolved.

Note: Glucose is highly soluble in water. It will dissolve quickly.

Step 2: Adjust the volume to 100 ml with deionized/Milli-Q water. Mix it again.

Step 3: Sterilize the solution by filtering it through a 0.20 µm membrane filter unit.

Precaution: 
Do not autoclave glucose solution as autoclaving can cause degradation of glucose.

Storage:
Store the solution in the cold room.

REFERENCES:
Woo et al., 2015. Characteristics of the Thermal Degradation of Glucose and Maltose Solutions. Prev Nutr Food Sci. 20(2), 102-9. PMID-26175997; Full-Text Link: PMC