Setting up Agarose Gel Electrophoresis in Laboratory

Agarose gel electrophoresis is a comparatively easy technique and requires limited knowledge & expertise, reagents, instruments and space.

KNOWLEDGE AND EXPERTISE
Pipetting: Handling micropipette is the major skill you need to load an accurate amount of your samples onto the wells of agarose gel without damaging the gel.
Handling of samples: You must have knowledge of working with nucleic acids, especially RNA as it is prone to degrades. 
Safety procedure: Some of the chemicals like nucleic acid staining dyes, formamide (for denaturing agarose gel electrophoresis) etc must be handled carefully. You must be aware of the hazardous effects of these chemicals on human health and environment, and must use appropriate procedures to handle and discard these chemicals. You must also know the hazardous effects of uv light on humans as you need this to visualize nucleic acids embedded in agarose gel.

EQUIPMENTS
Microwave oven/hot plate
Micropipette and tips
Regular glassware to prepare various solution and buffers
Fume hood (for denaturing agarose gel electrophoresis)
Gel Documentation system (to capture images of agarose gel)
Storage space in fridge and refrigerator
Electrophoresis apparatus, Casting tray, comb etc
Electric supply e.g., BioRad PowerPac TM

REAGENTS
Electrophoresis buffer
Agarose
Nucleic acid staining dyes
Loading dyes

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