TAE (Tris-Acetate-EDTA) Electrophoresis Buffer Calculator

Select TAE buffer concentration
Tris Base
Glacial acetic acid
Select EDTA Type *
* EDTA is available in several forms including anhydrous and EDTA disodium dihydrate form. Both forms can be used for the preparation of a TAE buffer. A 0.5M EDTA stock solution can also be used for the preparation of a TAE buffer. Remember that EDTA form has an effect on heating of electrophoresis buffer during electrophoresis with EDTA stock solution (0.5 M EDTA solution) causing highest heating effect and least heating with anhydrous EDTA (most difficult to dissolve).
Fill the desired volume in the box

(Change the value)

To prepare 1000 ml of 1 x TAE buffer, weigh out

4.24 g Tris base (C4H11NO3, Molecular Weight: 121.14),

1. 142 ml Glacial acetic acid (CH3COOH, Molecular Weight: 60.05) and

0.29 g of Anhydrous EDTA

and dissolve them in deionized/distilled water to a final volume 1000 ml

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