Tag: ags cell line
SupEvidence: CagA Induces EMT-Like Changes in AGS Cells
Helicobacter pylori is a gastric pathogen closely associated with the development of gastric cancer. One of its major virulence factors,…
Effect of CagA on β-Catenin Signaling in AGS Cells
Although AGS cells do not express functional E-cadherin, Helicobacter pylori can still modulate β-catenin signaling in these cells through E-cadherin–independent…
E-cadherin Expression in AGS Cell Line
AGS cells, a widely used human gastric adenocarcinoma cell line, do not express functional E-cadherin due to epigenetic silencing of…
β-Catenin Expression in AGS Cell Line
In normal epithelial cells, β-catenin is sequestered at the adherens junctions via interaction with E-cadherin, helping maintain junctional integrity and…
AGS Cell Line: Culture Media
Various culture media have been used to maintain the AGS cell line in culture and for specific experimental procedures. A 10% fetal bovine serum (FBS)-supplemented RPMI medium is the most popular medium used by many researchers to maintain AGS cells in culture. Some have also used 10% FBS-supplemented DMEM. ATCC suggests Ham’s F12K Medium supplemented with 10% FBS. A list of base media used for AGS cell line, experiment-specific supplements, culture conditions, purpose, and references are given in the table below.